Hypoxia, Blood Vessels, and Proliferation
Hypoxia, Blood Vessels and Cellular Proliferation in Human Skin: Images of skin from one patient presented to show the relationship between EF5 binding (red) and blood vessels (green; CD31; figure A) or proliferating cells (green; Ki67 - figure B). Blue regions are viable nuclei (Hoechst 33342). Notice that the proliferating cells (figure B) are in physiologically oxic to modestly hypoxic regions explaining the sensitivity of these cells to radiation. Blood vessels (figure A) are not seen within hypoxic regions but are consistently in an inverse location. This pattern and the distances between hypoxia and vessels is compatible with the accepted range of oxygen diffusion (150-300μ) for normally metabolizing tissues (Thomlinson and Gray, 1955).