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- Hoechst 33342
- Make fresh each week of thaw from frozen stock, keep out of light.
- MW is 561, to make 5mM solution, dissolve @ 2.8mg.ml in normal saline.
- For tissue sections or cells in suspension, aim for final concentration of 20µM which should stain fixed cells immediately. Make via: 80µl of 5mM Hoechst, plus 2.0 ml of 10xPBS + 18ml of deionized water. Dip slides directly into this solution in Coulter vial for 5min, followed by rinse in PBS.