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  • To a 50ml tube add 1.6ml of fat-free milk (made by dissolving 1g of Carnation skim milk powder in 9g of PBS.)
  • Add 0.4ml of desired serum (mouse, rat, goat, etc.).
  • Add 6ml of antibody carrier.
  • Refrigerate and store for no more than 2-3 weeks.

Comments? Questions? E-mail kochc@mail.med.upenn.edu