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Methods
EF5 Procedure
Materials
10x PBS
1000x Poisons
4% Paraformaldehyde
1X PBS
1X ttPBS
10mM EF5
Competed ttPBS
Antibody Carrier
Block
Normal Saline
Hoechst 33342
5-bromo-2’deoxyuridine
Peroxidase Reagents
ELK series Antibodies
In Vivo Use
Antibody Carrier
- Antibody Carrier
- Add 100ml of ttPBS to beaker with stirring bar.
- Slowly add 1.5g of lipid-fee albumin while continuing to stir.
- Refrigerate.
Comments? Questions? E-mail kochc@mail.med.upenn.edu