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1X ttPBS

    1X ttPBS (1XPBS with 0.3% tween 20)
  • On a macro balance weigh 16g of tween 20 (polyoxyethylene-sorbitan monolaurate) into a 4L beaker.
  • Measure 1800mL of filtered water and add about half to 4L beaker.
  • Add 400ml of 10XPBS, stir continuously.
  • Add 0.16g thimerosal, rinse weighing boat with some of remaining water .
  • Add 0.5g of sodium azide, rinse weighing boat with remaining water.
  • Add 1800ml more of filtered water and continue to stir.
  • Measure the pH using pH meter. Should be between 7.2-7.5. Refrigerate.

Comments? Questions? E-mail kochc@mail.med.upenn.edu