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Methods
EF5 Procedure
Materials
10x PBS
1000x Poisons
4% Paraformaldehyde
1X PBS
1X ttPBS
10mM EF5
Competed ttPBS
Antibody Carrier
Block
Normal Saline
Hoechst 33342
5-bromo-2’deoxyuridine
Peroxidase Reagents
ELK series Antibodies
In Vivo Use
1X PBS
- 1X PBS
- Measure 1800mL of filtered water and add about half to 4L beaker.
- Add 400ml of 10XPBS, stir continuously.
- Add 0.16g thimerosal, rinse weighing boat with some of remaining water .
- Add 0.5g of sodium azide, rinse weighing boat with remaining water.
- Add 1800ml more of filtered water and continue to stir.
- Measure the pH using pH meter. Should be between 7.2-7.5. Refrigerate.
Comments? Questions? E-mail kochc@mail.med.upenn.edu