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10x PBS

10x PBS (Dulbecco's phosphate buffered saline) with 100µM thimerosal

  • Slowly add 96.5g of Sigma D-5773 to 950ml of filtered water while stirring continuously in a calibrated 1L beaker.
  • Add 0.041g of Sigma T-5125 (thimerosal). Use care, toxic chemical.
  • Sodium Azide?
  • Top off beaker to the 1L level.
  • Filter

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