A number of imaging markers which target hypoxia exist and are widely used in current research. The current focus of this website however is on the use of EF5, a compound developed at the University of Pennsylvania by Dr. Cameron Koch and Dr. Sydney Evans.
One of the most pertinent benefits of the use of EF5 over similar hypoxia markers is that the resultant images can be calibrated according to camera settings and a "cube-binding" value which is obtained through a separate procedure. The intensity values of calibrated images are directly related to actual tissue pO2 values. As a result, these images provide information regarding not only where hypoxic areas may or may not be, but also data regarding the level of hypoxia.
For more information, please visit our references section.